FORM FOR THE ACCESSION OF PLANT CELL CULTURES

LABORATORY PROCEDURES FOR PLANT CELL LINES

Appendix

PC/1998/2/2.2 Appendix 1

TITLE: FORM FOR THE ACCESSION OF PLANT CELL CULTURES

1. PASSPORT DATA

1.1. CELL CULTURE NAME

Botanical Name of Initial Plant:

Strain Designation:

1.2. DEPOSITOR

Name of Depositor:

Institute:

Department:

Address:

Phone number: Fax Number:

Email:

1.3. TAXONOMIC NAME OF INITIAL PLANT

Genus name:

Author:

Species name:

Author:

Subspecies name:

Author:

Variety name:

Author:

Cultivar name:

Author:

other designation

Author:

Synonymous names:

(please use additional page if there is more than one synonymous name)

1.4. CELL CULTURE HISTORY:

Former institutes and periods of maintenance:

Origin of initial plant material:

Reference (Seed or Herbarium Number):

1.5. CELL CULTURE INITIATION

Material for culture initiation:

Method for surface sterilization of initial plant material:

In case of seeds: conditions for germination:

Cell culture initiator: Date of sterilization:

Date of first callus development: Date of culture establishment

Medium of callus initiation:

Method for the initiation of suspension culture:

Applied selection procedure for culture initiation or maintenance:

2. DATA FOR CULTURE MAINTENANCE

2.1. GROWTH CONDITIONS

Growth temperature:

Light conditions:

Rel. humidity:

Other requirements:

Growth medium:

Composition of medium (use additional sheet if necessary):

pH of medium and pH adjustment:

Sterilization temperature: Sterilization time:

Filter type for sterile filtration:

2.2. CULTURE HANDLING

Growth vessel:

Amount of Medium:

Transfer Period:

Transfer method (incl. amount of inoculum in case of suspension cultures):

Shaking (rpm):

Selection method applied for cell culture maintenance:

Method for Growth Measurement:

Method for Viability Measurement:

Remarks (any remarkable procedure for the medium preparation like: preparation of special stock solutions etc. or for the handling of cultures):

3. MORPHOLOGICAL CHARACTERISTICS

Growth (0 = very bad, 1 = bad, 2 = good, 3 = very good):

Consistency (from -2 = very soft to +2 = very rigid):

Colour :

Morphology:

Differentiated structures (embryos, shoots, roots):

4. SPECIAL TRAITS OF THE CULTURE

(give any metabolite or physiological capacity of this specific culture)

5. BRIEF DESCRIPTION OF A CRYOPRESERRVATION METHOD

(give only methods that have been successfully applied to this specific culture)

6. SAFETY AND REGULATORY CLASSIFICATION

Is the culture free of fungi: ( ) yes ( ) no ( ) not tested

bacteria: ( ) yes ( ) no ( ) not tested

viruses: ( ) yes ( ) no ( ) not tested

Which safety classification does the culture belong to (genetically transformed cultures may not be maintained at the moment):

L1: ( ) L2: ( )

S1: ( ) S2: ( )

Restrictions for the delivery of cell cultures:

The culture may be delivered without any restrictions: ( )

The culture may be delivered for research purposes only: ( )

Date: Signature:

7. REFERENCES

8. LIST OF ATTACHEMENTS

Guidelines prepared for CABRI by DSMZ, 20 Jan. 1998
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Copyright CABRI, 1998

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